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2016|17 Annual Report Fraunhofer IGB - Microbial production of basic chemicals and scale-up of fermentation processes

140 120 100 80 60 40 20 0 0 L / g n i d i c a c i n o c a t i 5 OH O OH 4 O OH Malic and itaconic acid Contact Fungal systems have proven to be particularly advantageous for the microbial production of malic acid and itaconic acid. Our research on fermentation development for the production of malic acid from C5 sugars of hemicellulose fractions is one example of the use of fungi. We establish biotechnological production of malic acid with Aspergillus strains that have the GRAS (Generally Recognized as Safe) status, an approval designation of the US FDA (Food and Drug Administration) indicating the safety of a substance as food additive. By us- ing product process optimization we were able to increase product concentration by a factor of 1.5 compared to what is reported in the literature. The fermentative production process of itaconic acid dates back to 1932, at that time using Aspergillus itaconicus. Now, the Aspergillus terreus strain is primarily used. We were able to yield 137 g / L itaconic acid using A. terreus for biotechno- logical applications. Biosurfactants Fraunhofer IGB has many years of expertise in the microbial production of biosurfactants. Different smut fungi of the genera Ustilago sp. and Pseudozyma sp. naturally secret the biosurfactants mannosylerythritol lipid (MEL) and cellobiose lipid (CL), which can be extracted from the culture medium. Application studies for different MEL and CL variants showed a broad application potential – as detergents, emulsii ers and active ingredients in cosmetics, crop protection products and for technical applications. Currently, we achieve product con- centrations of 30 g / L for CL and 100 g / L for MEL by optimiz- ing the production process. itaconic acid 2 4 6 8 10 12 14 time in d Dr.-Ing. Susanne Zibek Phone +49 711 970-4167 susanne.zibek@igb.fraunhofer.de Funding The projects “BioQED – Quod Erat Demonstrandum: Large scale demonstration for the bio-based bulk chemicals BDO and IA aiming at cost reduction and improved sustainability” and “BioConSepT” have received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613941 and grant agreement no 289194, respectively. Furthermore, we would like to thank the German Federal Ministry of Education and Research (BMBF) and the Baden- Württemberg-Stiftung for funding the project “KomBiChemPro”, promotional reference 031B0083A, and the German Federal Ministry of Food and Agriculture (BMEL), represented by the Agency for Renewable Resources (Fachagentur Nachwachsende Rohstoffe e. V., FNR) for funding the CLIB 2021 (Cluster Industri- elle Biotechnologie e. V.) project “PolyTe”, promotional reference 22012608. 1 Production of different long-chain dicarboxylic acids from fatty acids or second generation oils. 2 Aspergillus terreus in the production phase of itaconic acid production. 3 Production of different MEL and CL variants from renewable raw material oils. 4 Fermentative production of L-malic acid. 5 Course of itaconic acid production with Aspergillus terreus. Concentrations of 137 g / L can be achieved at shake l ask scale through process optimization. 8 9

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