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ePaper created 2017-03-14, 10:25:37 | version 1.49.03
2 100 μm Contact Outlook The data we generated in vitro suggest that the iron oxide nanoparticle prototype M4E is suitable for biocompatible labeling of MSCs and for non-invasive detection using MPI. If stem cells are cultured in a tissue-like 3D environment, labeling lasts for up to ive times longer than in conventional 2D cell culture. If we had carried out our study solely in a 2D environment, this would have yielded results that might not have corresponded to the situation in vivo. We will verify the in vitro / in vivo correlation of our data in subsequent animal tests. Furthermore, the 3D scaffold to which the labeled stem cells were applied could be used as an implant for regenera- Dr. rer. nat. Maria Steinke Phone +49 931 31-80720 maria.steinke@igb.fraunhofer.de Prof. Dr. hum. biol. Heike Walles Phone +49 931 31-88828 heike.walles@igb.fraunhofer.de Literature [1] Kilian, T.; Fidler, F.; Kasten, A.; Nietzer, S.; Landgraf, V.; Weiß, K.; Walles, H.; Westphal, F.; Hackenberg, S.; Grüttner, C.; Steinke, tive therapies, in which stem cells can differentiate or migrate to the target tissue. M. (2016) Stem cell labelling with iron oxide nanoparticles: im- pact of 3D culture on cell labelling maintenance. Nanomedicine (Lond) 11(15):1957 – 70. doi: 10.2217/nnm-2016-0042 Funding The project “IDEA – Identiication, homing and monitoring of therapeutic cells for regenerative medicine – Identify, Enrich, Accelerate” has received funding from the European Union’s Sev- enth Framework Programme for research, technological develop- ment and demonstration under grant agreement no 279288. Project partners Dr. Florian Fidler, Würzburg | Dr. Cordula Grüttner, Warne- münde | Priv.-Doz. Dr. Stephan Hackenberg, Würzburg | Dr. Annika Kasten, Rostock 5 5 1 Schematic of the particle prototype M4E. 2 Prussian blue staining of labeled stem cells. Particles are shown in blue.