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ePaper created 2017-03-14, 10:25:37 | version 1.49.03
50 μm 2 50 μm 3 predominantly occurs in sun-exposed skin areas like the head Contact and neck, forehead, nose, lips and hands with actinic keratosis being the most common form. As a pre-cancerous form (precursor), an invasive squamous cell carcinoma will develop if left untreated. In addition to surgical treatments, numerous non-surgical procedures, including photodynamic therapy (PDT) are available. By integrating white skin cancer cells (cell line SCC-25) into our well-established three-dimensional skin model simultaneously with healthy keratinocytes in deined ratios, we can mimic early and late stages of squamous cell carcinoma in humans, which are morphologically comparable to the in vivo situation. Using Raman micro-spectroscopy, we are able to non-destruc- tively distinguish healthy keratinocytes from tumor cells. Dipl.-Biol. (t.o.) Sibylle Thude Phone +49 711 970-4152 sibylle.thude@igb.fraunhofer.de Prof. Dr. rer. nat. Petra Kluger Phone +49 711 970-4072 petra.kluger@igb.fraunhofer.de Outlook Literature These models are advanced tools to monitor disease progres- [1] Biological evaluation of medical devices – Part 5: Tests for in sion and allow the investigation of new photosensitizing substances, adapted irradiation protocols with speciic light sources and their effects on healthy and diseased cells [4]. In collaboration with the University of Stuttgart, we have also developed a model for melanoma skin cancer. vitro cytotoxicity (ISO 10993-5:2009); German version EN ISO 10993-5:2009 [2] OECD guideline for the testing of chemicals, section 4, Health Effects: In vitro 3T3 NRU phototoxicity test (2004). DOI: 10.1787 / 9789264071162-en [3] Thude, S.; Kluger, P.; Schenke-Layland, K. (2015) In vitro- Hauttestsysteme zur Untersuchung lichtassoziierter Hautschädi- gung. Biospektrum 02.15, 21. Jahrgang: 172 – 174. DOI: 10.1007 / s12268-015-0557-z. Springer-Verlag. [4] Brauchle, E.; Johannsen, H.; Nolan, S.; Thude, S.; Schenke- Layland, K. (2013) Design and analysis of a squamous cell carcinoma in vitro model system. Biomaterials 30: 7401 – 7407. DOI:10.1016/j.biomaterials.2013.06.016 Funding We would like to thank the Fraunhofer-Gesellschaft for funding 1 In vitro skin – from basic to advanced models. the project “SkinCancer” within the scope of its SME-oriented 2 Effect of phototoxic substance before (top) and internal research program (MEF). after (bottom) UV-A-irradiation (5J / cm2). 3 UV-irradiation of pigmented skin models with the BioSUN. 5 3