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ePaper created 2014-05-07, 13:23:14 | version 1.30.01
81 Priv.-Doz. Dr. Susanne Bailer Phone +49 711 970-4180 susanne.bailer@igb.fraunhofer.de Priv.-Doz. Dr. Steffen Rupp Phone +49 711 970-4045 steffen.rupp@igb.fraunhofer.de Funding We would like to thank the German Federal Ministry of Educa- tion and Research (BMBF) for funding the project “FYI – Fungi Yeast Identification”, promotional reference 01EZ1113F. Project partners Euroimmun Medizinische Labordiagnostika AG, Lübeck (coor- dinator) | Heart and Diabetes Centre NRW, Bad Oeynhausen | IGVP, University of Stuttgart | Multi Channel Systems MCS GmbH, Reutlingen | Robert Bosch GmbH, Gerlingen developed for a number of genes that show highly conserved areas, but are also variable enough to enable discrimination between the species with DNA probes. The time required is currently 3 to 4 hours from the isolation of the DNA to de- tection on the array. Parallel to this, LOC systems, in which the DNA microarrays that we developed are currently being integrated, have been developed by our project partners. LOC-compatible cell disruption techniques are being worked out here in cooperation with our partners for different patient probes, such as respiratory secretions for the diagnosis of pa- tients with cystic fibrosis. In this context, the use of disposable cartridges makes the system flexible and economical. Outlook As a miniature laboratory, the LOC combines sample prepara- tion with highly sensitive and rapid molecular biological diag- nostics of yeasts and mold fungi directly on the chip – with high specificity and sensitivity. This enables them to support clinicians in their diagnoses and facilitates the rapid and ad- equate initiation or adjustment of therapy. The LOC system is designed such that it can be adapted for further sample materials, such as biopsy tissues or bacterial pathogens. Upon completion, the LOC will be introduced to the market by the partners. 1 Candida spp., dangerous pathogen for immunocompromized patients. 2 DNA microarray. 3 Time and process diagram for pathogen detection. 3 Patient sample (blood, BAL) t = 0 h Isolation and purification of fungal DNA t = 0.5 h Amplification of diagnostic target regions t = 3 h 1 2 3 4 5 MICROARRAY Hybridization of Cy3-PCR-product on FYI-Array t = 4 h Scanning and reliable identification of human pathogenic species t = 5 h Contacts