2012|13 Annual Report Fraunhofer IGB

93 OH O H HO H H OHH O O OHH O H HO H OH HO O HO O O OH OH R CL 100 μm Ac Ac OH O O O O O OH OH OH H H H H O O R R = C11 – C17 MEL Dipl.-Biol. (t. o.) Dipl.-Ing. (FH) Susanne Zibek Phone +49 711 970-4167 susanne.zibek@igb.fraunhofer.de Priv.-Doz. Dr. Steffen Rupp Phone +49 711 970-4045 steffen.rupp@igb.fraunhofer.de References Günther, M.; Zibek, S. ; Hirth, T. ; Rupp, S. (2010) Synthese und Optimierung von Cellobioselipiden und Mannosylerythritollipi- den. Chem. Ing. Tech. 82: 1215–1221 Funding We would like to thank the German Federal Ministry of Educa- tion and Research (BMBF) for funding the project “BioSurf – Novel production strategies for biosurfactants” via the ERA-NET Industrial Biotechnology 2nd Joint Call (ERA-IB), promotional ref- erence 0315928A, ERA-IB10.039. Project partners Karlsruher Institut für Technologie KIT, Karlsruhe | c-LEcta GmbH, Leipzig | Flemish Institute for Technological Research, Mol, Belgium | Tormans Engineering Noord BVBA, Geel, Belgium | Ecover Belgium NV, Malle, Belgium | LISBP, Toulouse, France Further information www.biosurf.de Biosurfactant variants for application tests Depending on the microorganism the MEL and CL produced come in different variants. In order to be able to evaluate as many of these variants as possible, 11 strains have been tested to date as to their product range. Currently we are producing sample substances of MEL and CL at a small scale for applica- tion tests within the consortium. Product concentrations for MELs reached up to 100g/L and for CL up to 33 g/L. Enzymatic optimization and metabolic engineering For the creation of further surfactant variants a MEL blend generated from P. aphidis was deacetylized using a lipase. The product generated was tested as to its surfactant effect. Various structural variants with modiﬁed surfactant proper- ties were also produced from cellobiose lipids. By carrying out genome-wide studies on a particularly efﬁcient MEL producer using Next Generation Sequencing, we were able to iden- tify the genes required for MEL biosynthesis. These data will now serve as a blue print for the metabolic engineering of the strain with the aim of obtaining MEL variants with tailor-made properties. Future work will focus on the scale-up of the fermentation process to a cubic meter scale and the relevant downstream processes. 3 4 1 Some biosurfactants are good foaming agents. 2 Fermentative synthesis of cellobiose lipids in the 1-liter bioreactor. 3 At high product concentrations, mannosylerythritol lipids (MEL) are deposited as oily pearls. 4 Cellobiose lipids (CL) as needle-shaped crystals. R=H or OH Contacts

2012|13 Annual Report Fraunhofer IGB

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