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ePaper created 2013-09-24, 11:56:35 | version 1.25.20
77 Anke Hoppensack M.Sc. Phone +49 711 970-4073 anke.hoppensack@igb.fraunhofer.de Dr.-Ing. Jan Hansmann Phone +49 711 970-4084 jan.hansmann@igb.fraunhofer.de References [1] Dankers, P. Y. W.; Boomker, J. M.; Meijer, E. W.; Popa, E. R.; van Luyn, M. J. A. (2011) From kidney development to drug deliv- ery and tissue engineering strategies in renal regenerative medi- cine, Journal of Controlled Release 152(1): 177–85 [2] Tasnim, F.; Deng, R.; Hu, M.; Liour, S.; Li, Y.; Ni, M.; Ying, J. Y.; Zink, D. (2010) Achievements and challenges in bioartificial kid- ney development, Fibrogenesis Tissue Repair 10: 3–14 [3] Colter, D. C.; Seyda, A.; Buensuceso, C. S.; Gosiewska, A. (2008) Kidney-derived cells and methods of use in tissue repair and regeneration, Ethicon Inc (Somerville, NJ, US) US Patent 20080112939 Project partner The presented results were generated in cooperation of the Fraunhofer IGB together with the company Advanced Technolo- gies and Regenerative Medicine (ATRM), LLC, a Johnson & John- son company, Somerville, NJ, USA. Moreover, Jan Hansmann had the possibility to do research studies in the laboratories of the project partner. We thank ATRM for the support and cooperation in this project. The investigation of cell functionality showed that after 3 days in culture, hKDCs started to generate a glucose concentra- tion gradient (Fig. 5). Even after impressed equilibration of the concentrations, hKDCs restored the initial concentration dif- ference within 5–6 hours. hKDCs glucose transport function- ality was tested over 3 weeks. The simulations demonstrated a high consistency in the measured values and calculated results. The mathematical model allowed deriving the mag- nitude of active glucose mass transport of hKDCs in culture. Furthermore, a first draft for a proximal tubulus system could be developed Perspective With the generated data, bioreactor and extracorporeal proxi- mal tubule device design, crucial fundamentals for the devel- opment of novel bioartificial kidney assist devices were set. Employing standard filter technology, the generated filtrate could be fed into the bioartificial proximal tubule to recover molecules back into the patient’s blood. In further studies, the system needs to be experimentally validated and further char- acterized to facilitate the translation of the results into a clini- cal system. 1 hKDCs cultured on a synthetic membrane (top) and on a biological matrix (bottom). 2 Bioreactor system for proximal tubule specific cell culture. 3 Mass transport pathways. 4 Parameter estimation. 5 Apical and basolateral glucose concentrations during hKDC culture. 4 5 Contacts 0 Glucoseconcentration[mg/ml] Day 0 Day 3 Day 7 Day 10 Day 14 Day 18 Day 21 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 Glucoseconcentration[kg/m3 ] Time [s] 0 1000 2000 3000 4000 5000 1.8 1.7 1.6 1.5 1.4 1.3 1.2 Measured concentration apical Measured concentration basolateral Calculated concentration basolateral Calculated concentration apical