2012|13 Annual Report Fraunhofer IGB

Contact Priv.-Doz. Dr. Steffen Rupp Head of Department of Molecular Biotechnology Phone +49 711 970-4045 steffen.rupp@igb.fraunhofer.de Range of services Screening of targets and active compounds for anti- infectives (2D and LC proteomics, DNA microarrays, parallel sequencing, infection models, screening assays) Gene expression analyses and genome sequencing for customers Development of DNA microarrays: design of probes, production of PCR fragments, contact printing, and hybridization Cell-based assays: antiviral assays (GLP), pyrogen detection, mutagenicity, toxicity Development of production cell lines and processes for recombinant production of proteins (biosimilars), protein puriﬁcation and characterization Development of high-throughput enzyme assays and screening Strain and parameter screening in multi-fermenter systems Development of integrated fermentation processes for industrial biotechnology with a focus on downstream processing of raw materials and products Chemical-physical and biochemical analysis Infrastructure and technical equipment Molecular-biological laboratories conforming to safety levels L2, S1 and S2 of the German GenTSV (genetic engi- neering safety regulations) Microarray facility, universal microarray platform Quantitative real time PCR (qRT-PCR LightCycler 480) Parallel sequencing facility (Illumina HiSeq GAIIx) Proteomics facility using high-resolution MS techniques (2D gel electrophoresis, nano-LC-MALDI-TOF/TOF, HPLC- ESI-MS/MS) Fermentation plant for suspension and adherent mammali- an cell culture up to 10 L (non-GMP) Protein puriﬁcation equipment Pulping machines (ball mills, etc.), multi-fermentation bio- reactors for bioprocess development, and small bioreac- tors (up to 30 L) S2 Picking robot for the systematic storage of DNA- and mi- crobial libraries Accredited analytical lab: GC-MS/MS, LC-MS/MS, GPC, IC, ICP-AES and ICP-MS 45

2012|13 Annual Report Fraunhofer IGB

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