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77 Dr. sc. hum. Marco Metzger Phone +49 931 31-86686 marco.metzger@igb.fraunhofer.de Dr. rer. nat Maria Steinke Phone +49 931 31-80720 maria.steinke@igb.fraunhofer.de Contact Literature [1] Steinke, M.; Gross, R.; Walles, H.; Gangnus, R.; Schütze, K.; Walles, T. (2014) An engineered 3D human airway mucosa model based on an SIS scaffold Biomaterials 35: 7355–62 [2] WHO Factsheet N°259; http://www.who.int/mediacentre/ factsheets/fs259/en/ Funding We would like to thank the Free State of Bavaria (BayernFIT- Programm) and the Interdisciplinary Center for Clinical Research IZKF of the medical faculty of the University of Würzburg for funding our work. Project partners Prof. Dr. Thorsten Walles, Department of Thoracic and Cardio- vascular Surgery, University Hospital of Würzburg | Prof. Dr. Roy Gross, Chair of Microbiology, University of Würzburg | Prof. Dr. Markus Engstler, Chair of Cell and Development Biology, University of Würzburg | Dr. Cynthia Sharma, Research Center for Infectious Diseases, University of Würzburg | Prof. Dr. Jörg Vogel, Institute for Molecular Infection Biology, University of Würzburg intestinal epithelium (Caco-2), the blood barrier (endothelial cells), and the components of the immune system (Peripheral Blood Mononuclear Cells, PBMCs). By means of Salmonellae marked with fluorescence ig , the transmigration was e amined through flow cytometry s a result, a time-depen- dent increase of infected epithelial cells was shown while the endothelium was not affected. Moreover, the infection led to the release of IL-8 into the vascular compartment and an activation of monozytes (CD14+) and NK-cells (CD56+). In a second cooperation project (ZINF, Dr. Cynthia Sharma’s workgroup), we were able to integrate the mucigeneous cell line E12 (HT29-MTX-E12, Fig. 4 below). After the infection with Campylobacter it was shown that the mucosa represents an additional barrier with respect to colonization, transmigra- tion, and destruction of the epithelial conglomerate. Overview Understanding important steps of a natural infection forms the basis for developing new preventive and therapeutic strat- egies for the fight against infectious diseases Our in vitro test systems are suitable to further investigate these infection mechanisms and can support the (further) development of therapy strategies and vaccines in the long run. 3 1 3D test system of the human airway mucosa. 2 Tsetse fly during the infection of an in vitro skin model. 3 Pathogen of sleeping sickness beside a human cell. 4 Infections with Salmonella or Campylobacter spec. on Caco-2 resp. E12 intestine models. 4 CampylobacterSalmonella Phone +4993131-86686 Phone +4993131-80720